I know this thread is ancient, but it's still one of only a handful of threads on the web talking about this issue, and nobody has ever posted a solution publicly that I can see, so I'm mega-bumping it in the interests of posterity. If you have a Sega System 24 arcade PCB, and you want to know how to copy or backup the floppy disks from a PC, or if you want to be able to create a floppy from the MAME disk images, or re-create the MAME disk images from an original disk, I've put instructions up on the web here: http://nemesis.exodusemulator.com/Arcade/System24/System24Disks.html
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